* using log directory ‘/home/hornik/tmp/CRAN_special_donttest/ggpicrust2.Rcheck’ * using R Under development (unstable) (2026-02-10 r89394) * using platform: x86_64-pc-linux-gnu * R was compiled by gcc-15 (Debian 15.2.0-12) 15.2.0 GNU Fortran (Debian 15.2.0-12) 15.2.0 * running under: Debian GNU/Linux forky/sid * using session charset: UTF-8 * current time: 2026-02-11 15:52:38 UTC * using option ‘--run-donttest’ * checking for file ‘ggpicrust2/DESCRIPTION’ ... OK * checking extension type ... Package * this is package ‘ggpicrust2’ version ‘2.5.8’ * package encoding: UTF-8 * checking CRAN incoming feasibility ... [6s/8s] NOTE Maintainer: ‘Chen Yang ’ Suggests or Enhances not in mainstream repositories: Maaslin2 Availability using Additional_repositories specification: Maaslin2 no ? ? ? https://bioconductor.org/packages/release/bioc * checking package namespace information ... OK * checking package dependencies ... INFO Package suggested but not available for checking: ‘Maaslin2’ * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for executable files ... OK * checking for hidden files and directories ... OK * checking for portable file names ... OK * checking for sufficient/correct file permissions ... OK * checking whether package ‘ggpicrust2’ can be installed ... [7s/8s] OK * checking package directory ... OK * checking for future file timestamps ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... OK * checking top-level files ... OK * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking code files for non-ASCII characters ... OK * checking R files for syntax errors ... OK * checking whether the package can be loaded ... [1s/1s] OK * checking whether the package can be loaded with stated dependencies ... [1s/1s] OK * checking whether the package can be unloaded cleanly ... [1s/1s] OK * checking whether the namespace can be loaded with stated dependencies ... [1s/1s] OK * checking whether the namespace can be unloaded cleanly ... [1s/1s] OK * checking loading without being on the library search path ... [1s/1s] OK * checking whether startup messages can be suppressed ... [1s/1s] OK * checking use of S3 registration ... OK * checking dependencies in R code ... OK * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... OK * checking R code for possible problems ... [12s/12s] OK * checking Rd files ... [0s/0s] OK * checking Rd metadata ... OK * checking Rd line widths ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... OK * checking for code/documentation mismatches ... OK * checking Rd \usage sections ... OK * checking Rd contents ... OK * checking for unstated dependencies in examples ... OK * checking contents of ‘data’ directory ... OK * checking data for non-ASCII characters ... [1s/1s] OK * checking LazyData ... OK * checking data for ASCII and uncompressed saves ... OK * checking installed files from ‘inst/doc’ ... OK * checking files in ‘vignettes’ ... OK * checking examples ... [9s/9s] ERROR Running examples in ‘ggpicrust2-Ex.R’ failed The error most likely occurred in: > base::assign(".ptime", proc.time(), pos = "CheckExEnv") > ### Name: compare_daa_results > ### Title: Compare the Consistency of Statistically Significant Features > ### Aliases: compare_daa_results > > ### ** Examples > > ## No test: > library(magrittr) > library(ggpicrust2) > library(tibble) > data("metacyc_abundance") > data("metadata") > > # Run pathway_daa function for multiple methods > methods <- c("DESeq2", "edgeR","Maaslin2") > daa_results_list <- lapply(methods, function(method) { + pathway_daa(abundance = metacyc_abundance %>% column_to_rownames("pathway"), + metadata = metadata, group = "Environment", daa_method = method) + }) converting counts to integer mode Note: levels of factors in the design contain characters other than letters, numbers, '_' and '.'. It is recommended (but not required) to use only letters, numbers, and delimiters '_' or '.', as these are safe characters for column names in R. [This is a message, not a warning or an error] Note: levels of factors in the design contain characters other than letters, numbers, '_' and '.'. It is recommended (but not required) to use only letters, numbers, and delimiters '_' or '.', as these are safe characters for column names in R. [This is a message, not a warning or an error] gene-wise dispersion estimates mean-dispersion relationship -- note: fitType='parametric', but the dispersion trend was not well captured by the function: y = a/x + b, and a local regression fit was automatically substituted. specify fitType='local' or 'mean' to avoid this message next time. Note: levels of factors in the design contain characters other than letters, numbers, '_' and '.'. It is recommended (but not required) to use only letters, numbers, and delimiters '_' or '.', as these are safe characters for column names in R. [This is a message, not a warning or an error] final dispersion estimates Disp = 0.69705 , BCV = 0.8349 Error in require_package(method_packages[[daa_method]], purpose = daa_method) : Package 'Maaslin2' is required for Maaslin2. Install with: BiocManager::install('Maaslin2') Calls: lapply -> FUN -> pathway_daa -> require_package Execution halted * checking for unstated dependencies in ‘tests’ ... OK * checking tests ... [27s/27s] OK Running ‘testthat.R’ [27s/27s] * checking for unstated dependencies in vignettes ... OK * checking package vignettes ... OK * checking re-building of vignette outputs ... [1s/1s] OK * checking PDF version of manual ... [4s/4s] OK * checking HTML version of manual ... [3s/3s] OK * checking for non-standard things in the check directory ... OK * checking for detritus in the temp directory ... OK * DONE Status: 1 ERROR, 1 NOTE