test_that("Importing data from mgatk works", { set.seed(1) data.dir <- system.file("extdata", "test_mgatk", package="Signac") mgatk <- ReadMGATK(dir = data.dir) # We imported 11 cells expect_equal(object = dim(mgatk$depth), expected = c(11,1)) # We get + and - counts for every position expect_equal(object = dim(mgatk$counts), expected = c(8*dim(mgatk$refallele)[1],11)) }) test_that("Variant calling is operational", { data.dir <- system.file("extdata", "test_mgatk", package="Signac") mgatk <- ReadMGATK(dir = data.dir) var_df <- IdentifyVariants(mgatk$counts, mgatk$refallele) possible_vars_df <- var_df[var_df$strand_correlation > 0.65 & var_df$n_cells_conf_detected > 3,] homoplasmic_vars <- possible_vars_df[possible_vars_df$mean > 0.9,"variant"] heteroplasmic_vars <- possible_vars_df[possible_vars_df$mean < 0.5,"variant"] # We get the expected # of homoplasmic variants expect_equal(object = length(homoplasmic_vars), expected = 42) # We get the expected # of heteroplasmic variants expect_equal(object = length(heteroplasmic_vars), expected = 11) }) test_that("Allele frequency calculation works", { data.dir <- system.file("extdata", "test_mgatk", package="Signac") mgatk <- ReadMGATK(dir = data.dir) vars.compute <- c("627G>A", "1888G>A", "1888G>C") expected <- new("dgCMatrix", i = c(1L, 0L, 2L, 1L, 0L, 2L, 1L, 2L, 0L, 0L,1L, 1L, 0L, 2L), p = c(0L, 1L, 3L, 3L, 4L, 6L, 8L, 9L, 10L, 11L, 12L, 14L), Dim = c(3L, 11L), Dimnames = list(c("627G>A", "1888G>A", "1888G>C"), c("ACAGGCCGTGGTCGAA-1", "AAACTGCAGAGTCCGA-1", "AAAGATGCAATGGCTT-1", "ACTAGGTAGTGTCGGA-1", "ACCATCCTCTTGTCGC-1", "ACAGAAATCTATCTCA-1", "AATGGCTCAACGTAGG-1", "ACCAAACGTTGTGAGG-1", "AACCGATTCAAGAGAT-1", "ACTAGGTCAATGCCAT-1", "AAAGGGCAGCTACGCC-1")), x = c(0.0947368421052632, 0.0955631399317406, 0.00273972602739726, 0.108108108108108, 0.0833333333333333, 0.00409836065573771, 0.0256410256410256, 0.00233100233100233, 0.087431693989071, 0.13768115942029, 0.0308788598574822, 0.072, 0.0254237288135593, 0.00689655172413793), factors = list()) alleles <- AlleleFreq( object = mgatk$counts, variants = vars.compute ) expect_equal(object = alleles, expected = expected) })