data("GlobalPatterns", package = "phyloseq") data("enterotype") data_fungi_low_high <- subset_samples( data_fungi_mini, Height %in% c("Low", "High") ) data_basidio <- subset_taxa(data_fungi, Phylum == "Basidiomycota") data_fungi_2trees <- subset_samples( data_fungi, data_fungi@sam_data$Tree_name %in% c("A10-005", "AD30-abm-X") ) GlobalPatterns@sam_data[, "Soil_logical"] <- ifelse(GlobalPatterns@sam_data[, "SampleType"] == "Soil", "Soil", "Not Soil") GP_archae <- subset_taxa( GlobalPatterns, GlobalPatterns@tax_table[, 1] == "Archaea" ) GP <- subset_samples_pq( GP_archae, GP_archae@sam_data$SampleType %in% c("Soil", "Skin") ) res_deseq <- DESeq2::DESeq(phyloseq_to_deseq2(GP, ~SampleType), test = "Wald", fitType = "local") test_that("Test one case for each deprecated functions", { skip_on_cran() expect_warning(physeq_graph_test(data_fungi_mini, fact = "Tree_name"), "deprecated") expect_s3_class(suppressWarnings(adonis_phyloseq(data_fungi_mini, "Tree_name")), "anova") expect_s4_class(suppressWarnings(clean_pq(data_fungi_mini)), "phyloseq") expect_message(expect_warning(otu_circle(data_fungi_2trees, fact = "Tree_name", nproc = 1, add_nb_seq = FALSE), "deprecated")) expect_message(expect_warning(biplot_physeq(data_fungi_2trees, merge_sample_by = "Tree_name"), "deprecated")) testFolder <- tempdir() unlink(list.files(testFolder, full.names = TRUE), recursive = TRUE) expect_warning(write_phyloseq(enterotype, path = testFolder, silent = TRUE), "deprecated") expect_s4_class(suppressWarnings(read_phyloseq(testFolder, taxa_are_rows = TRUE)), "phyloseq") expect_warning(sankey_phyloseq(data_fungi_mini), "deprecated") expect_s3_class(suppressWarnings(summary_plot_phyloseq(data_fungi_mini)), "ggplot") expect_message(expect_warning(plot_edgeR_phyloseq(GlobalPatterns, c("SampleType", "Soil", "Feces"), color_tax = "Kingdom"), "deprecated"), "Perform edgeR binary test") expect_warning(plot_deseq2_phyloseq(res_deseq, c("SampleType", "Soil", "Skin"), tax_table = GP@tax_table, color_tax = "Kingdom"), "deprecated") expect_warning(venn_phyloseq(data_fungi_mini, "Height"), "deprecated") expect_warning(ggVenn_phyloseq(data_fungi_mini, "Height"), "deprecated") expect_warning(hill_tuckey_phyloseq(GlobalPatterns, "Soil_logical"), "deprecated") expect_message(expect_warning(hill_phyloseq(GP, "SampleType"), "deprecated")) library(metacoder) expect_warning(suppressMessages(ht <- physeq_heat_tree(data_basidio)), "deprecated") expect_s3_class(suppressWarnings(multiple_share_bisamples(data_fungi_low_high, bifactor = "Height", merge_sample_by = "Height")), "tbl_df") }) suppressWarnings(vsearch_error_or_not <- try(system("vsearch 2>&1", intern = TRUE), silent = TRUE)) if (class(vsearch_error_or_not) == "try-error") { message("lulu_phyloseq() can't be tested when vsearch is not installed") } else { test_that("lulu_phyloseq works fine", { expect_s4_class(suppressWarnings(lulu_phyloseq(data_fungi_sp_known)$new_physeq), "phyloseq") }) }